ARG51772
anti-MARCKS phospho (Ser158) antibody
anti-MARCKS phospho (Ser158) antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MARCKS phospho (Ser158) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MARCKS |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 158 (R-F-S(p)-F-K) derived from Human MARCKS. |
| Conjugation | Un-conjugated |
| Alternate Names | MACS; 80K-L; Myristoylated alanine-rich C-kinase substrate; PKCSL; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; MARCKS; PRKCSL |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MARCKS |
| Gene Full Name | myristoylated alanine-rich protein kinase C substrate |
| Background | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. |
| Function | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 32 kDa |
| PTM | Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. |
Images (2) Click the Picture to Zoom In
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ARG51772 anti-MARCKS phospho (Ser158) antibody WB image
Western blot: Extracts from 3T3 cells stained with ARG51772 anti-MARCKS phospho (Ser158) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
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ARG51772 anti-MARCKS phospho (Ser158) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51772 anti-MARCKS phospho (Ser158) antibody.
