ARG11133
anti-MARCKS antibody
anti-MARCKS antibody for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MARCKS |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MARCKS |
| Antigen Species | Human |
| Immunogen | Full-length recombinant Human MARCKS. |
| Conjugation | Un-conjugated |
| Alternate Names | MACS; 80K-L; Myristoylated alanine-rich C-kinase substrate; PKCSL; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; MARCKS; PRKCSL |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HEK293, HeLa and SH-SY5Y | ||||||
| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P29966 Human Myristoylated alanine-rich C-kinase substrate |
|---|---|
| Gene Symbol | MARCKS |
| Gene Full Name | myristoylated alanine-rich protein kinase C substrate |
| Background | The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008] |
| Function | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton. Membrane; Lipid-anchor. [UniProt] |
| Calculated MW | 32 kDa |
| PTM | Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG11133 anti-MARCKS antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG11133 anti-MARCKS antibody (red) at 1:1000 dilution, and co-stained with anti-beta Tubulin antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
The MARCKS antibody recognizes protein localized in the plasma membrane and cytoplasm, while the beta Tubulin antibody stains the network of cytoplasmic microtubules.
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ARG11133 anti-MARCKS antibody WB image
Western blot: HEK293, HeLa, SH-SY5Y, COS1, NIH/3T3 and C6 cell lysates stained with ARG11133 anti-MARCKS antibody (green) at 1:1000 dilution and ARG52320 anti-GAPDH antibody [1D4] (red) at 1:5000 dilution.
