ARG63069
anti-MAP2ab antibody [MT-08]
anti-MAP2ab antibody [MT-08] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Pig
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [MT-08] recognizes MAP2ab |
|---|---|
| Tested Reactivity | Hu, Ms, Pig |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The clone MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MT-08 |
| Isotype | IgG1 |
| Target Name | MAP2ab |
| Immunogen | Microtubule protein (bovine brain) enriched for kinesin |
| Conjugation | Un-conjugated |
| Alternate Names | MAP2A; Microtubule-associated protein 2; MAP2B; MAP-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||||
| Positive Control | WB, IP and ELISA: Porcine brain IHC-P: Brain IHC-Fr: Mouse brain ICC/IF: SH-SY5Y |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MAP2 |
| Gene Full Name | microtubule-associated protein 2 |
| Background | MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells. |
| Function | The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt] |
| Highlight | Related products: MAP2 antibodies; MAP2 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| Research Area | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody |
| Calculated MW | 200 kDa |
| PTM | Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity). |
Images (1) Click the Picture to Zoom In
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ARG63069 anti-MAP2ab antibody [MT-08] WB image
Western blot: Microtubules partially purified from porcine brain lysate stained with ARG63069 anti-MAP2ab antibody [MT-08].
