ARG41910

anti-MAP2 antibody

anti-MAP2 antibody for ICC/IF,Western blot and Human,Mouse

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes MAP2
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MAP2
Conjugation Un-conjugated
Alternate Names MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:500
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300, 20% Glycerol and 1% BSA.
Preservative 0.025% ProClin 300
Stabilizer 20% Glycerol and 1% BSA
Concentration 0.35 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17756 Mouse MAP2

GeneID: 4133 Human MAP2

Swiss-port # P11137 Human Microtubule-associated protein 2

Swiss-port # P20357 Mouse Microtubule-associated protein 2

Gene Symbol MAP2
Gene Full Name microtubule-associated protein 2
Background This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton. Cell projection, dendrite. [UniProt]
Highlight Related products:
MAP2 antibodies; MAP2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
Calculated MW 200 kDa
PTM Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity). [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG41910 anti-MAP2 antibody ICC/IF image

    Immunofluorescence: DBTRG-05MG cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with ARG41910 anti-MAP2 antibody (red) at 1:200 dilution and 4°C. DAPI (blue) for nuclear staining.

  • ARG41910 anti-MAP2 antibody WB image

    Western blot: 60 µg of Mouse brain and Mouse colon lysates stained with ARG41910 anti-MAP2 antibody at 1:500 dilution, overnight at 4°C.