ARG44178

anti-MAGED4 antibody

anti-MAGED4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes MAGED4
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MAGED4
Antigen Species Human
Immunogen Recombinant protein of Human MAGED4
Conjugation Un-conjugated
Protein Full Name Melanoma-associated antigen D4
Alternate Names MAGED4; MAGE Family Member D4; KIAA1859; MAGE-E1; MAGE1; Melanoma-Associated Antigen D4; Melanoma Antigen Family D4; MAGE-D4 Antigen; MAGE-E1 Antigen; MGC3210 MAGEE1; Melanoma Antigen Family D, 4; MAGE-D4; MAGED4A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg / 10^6 cells
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol MAGED4
Gene Full Name Mage Family Member D4
Background Predicted to be involved in negative regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.
Function May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
Cellular Localization Nucleus
Calculated MW 81 kDa

Images (4) Click the Picture to Zoom In

  • ARG44178 anti-MAGED4 antibody IHC-P image

    Immunohistochemistry: Human adrenocortical adenoma stained with ARG44178 anti-MAGED4 antibody at 2 μg/mL dilution.

  • ARG44178 anti-MAGED4 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG44178 anti-MAGED4 antibody at 5 μg/mL dilution.

  • ARG44178 anti-MAGED4 antibody WB image

    Western blot: HepG2 and U2OS stained with ARG44178 anti-MAGED4 antibody at 0.5 μg/mL dilution.

  • ARG44178 anti-MAGED4 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG44178 anti-MAGED4 antibody at 1 μg/1x10^6 cells dilution.