ARG63500
anti-MAD2L1 antibody
anti-MAD2L1 antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes MAD2L1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MAD2L1 |
| Antigen Species | Human |
| Immunogen | C-KVNSMVAYKIPVND |
| Conjugation | Un-conjugated |
| Alternate Names | Mitotic spindle assembly checkpoint protein MAD2A; Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1; MAD2; HsMAD2; HSMAD2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13257 Human Mitotic spindle assembly checkpoint protein MAD2A |
|---|---|
| Background | MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody |
| Calculated MW | 24 kDa |
| PTM | Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2. |
Images (1) Click the Picture to Zoom In
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ARG63500 anti-MAD2L1 antibody WB image
Western Blot: HEK293 lysate (35 µg protein in RIPA buffer) stained with ARG63500 anti-MAD2L1 antibody at 2 µg/ml dilution.
