ARG10025
anti-M-CSF antibody [21]
anti-M-CSF antibody [21] for ELISA and Human
Cell Biology and Cellular Response antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [21] recognizes M-CSF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Does not cross react with BSA or other human cytokines tested such as IL-1β, IL-8, IL-16, EGF, G-CSFR, GM-CSF, MCP-1, MCP-3, TGF-β and TNF-α. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 21 |
| Isotype | IgG2a, kappa |
| Target Name | M-CSF |
| Antigen Species | Human |
| Immunogen | Purified recombinant human M-CSF (rhM-CSF) |
| Conjugation | Un-conjugated |
| Alternate Names | Macrophage colony-stimulating factor 1; CSF-1; Lanimostim; M-CSF; MCSF |
Application Instructions
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P09603 Human Macrophage colony-stimulating factor 1 |
|---|---|
| Gene Symbol | CSF1 |
| Gene Full Name | colony stimulating factor 1 (macrophage) |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| Function | Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody |
| Calculated MW | 60 kDa |
| PTM | N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated. |
