ARG22863
anti-Ly 6C antibody [ER-MP20] (PE)
anti-Ly 6C antibody [ER-MP20] (PE) for Flow cytometry and Mouse
Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
Overview
| Product Description | PE-conjugated Rat Monoclonal antibody [ER-MP20] recognizes Ly 6C |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | ER-MP20 |
| Isotype | IgG2a |
| Target Name | Ly 6C |
| Antigen Species | Mouse |
| Immunogen | Balb/c macrophage precursor cell hybrids. |
| Conjugation | PE |
| Alternate Names | FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | Ly6c1 |
|---|---|
| Gene Full Name | lymphocyte antigen 6 complex, locus C1 |
| Background | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody |
| Calculated MW | 29 kDa |
| PTM | Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms. The soluble form is produced by a proteolytic cleavage. |
Images (1) Click the Picture to Zoom In
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ARG22863 anti-Ly 6C antibody [ER-MP20] (PE) FACS image
Flow Cytometry: Figure A. FITC conjugated Rat anti Mouse CD8 and PE-conjugated Rat IgG2a isotype control. Figure B. FITC conjugated Rat anti Mouse CD8 and ARG22863 anti-Ly 6C antibody [ER-MP20] (PE). All experiments performed on Mouse splenocytes.
