ARG62532
anti-Laminin S antibody [C4]
anti-Laminin S antibody [C4] for Immunoprecipitation,Western blot and Human,Rat,Bovine,Chicken,Guinea pig,Pig
Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [C4] recognizes Laminin S |
|---|---|
| Tested Reactivity | Hu, Rat, Bov, Chk, Gpig, Pig |
| Tested Application | IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C4 |
| Isotype | IgG1 |
| Target Name | Laminin S |
| Antigen Species | Bovine |
| Immunogen | bovine anterior lens capsule |
| Conjugation | Un-conjugated |
| Alternate Names | Laminin-3 subunit beta; S-laminin subunit beta; Laminin B1s chain; S-LAM beta; Laminin-4 subunit beta; LAMS; NPHS5; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-9 subunit beta; Laminin-11 subunit beta; Laminin-7 subunit beta; Laminin subunit beta-2 |
Application Instructions
| Application Note | IP: 2 µg/mg of lysate. WB: 1 - 2 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | LAMB2 |
| Gene Full Name | laminin, beta 2 (laminin S) |
| Background | Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 2. The beta 2 chain contains the 7 structural domains typical of beta chains of laminin, including the short alpha region. However, unlike beta 1 chain, beta 2 has a more restricted tissue distribution. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported. It was suggested that inefficient splicing of this first intron, which does not change the protein sequence, results in a greater abundance of the unspliced form of the transcript than the spliced form. The full-length nature of the spliced transcript is not known. [provided by RefSeq, Aug 2011] |
| Cellular Localization | Basement membrane |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 196 kDa |
Clone References
Characterization of commercial laminin preparations from human placenta in comparison to recombinant laminins 2 (alpha2beta1gamma1), 8 (alpha4beta1gamma1), 10 (alpha5beta1gamma1).
WB / Human
