ARG10736
anti-Laminin 111 antibody
anti-Laminin 111 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Publication2
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Laminin 111 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Specificity | ARG10736 Laminin-111 antibody recognizes 3 laminin isotypes: α1 (440kDa), β1 (220kD) and γ1 (220kDa). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Laminin 111 |
| Antigen Species | Mouse |
| Immunogen | Laminin isolated from Mouse EHS cells. |
| Conjugation | Un-conjugated |
| Alternate Names | LAMA; PTBHS; S-LAM-alpha; Laminin subunit alpha-1; laminin, beta 1; laminin, gamma 1 |
Application Instructions
| Application Suggestion |
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| Application Note | Note: Based on the reference PMID: 35453580 This antibody may also suitable for IHC-P application in human samples. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Lama1 |
| Gene Full Name | laminin, alpha 1; laminin, beta 1; laminin, gamma 1 |
| Background | This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014] |
| Function | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. [UniProt] |
| Cellular Localization | Secreted › extracellular space › extracellular matrix › basement membrane Note: Major component. |
| Calculated MW | 337 kDa |
| PTM | Tyrosine phosphorylated by PKDCC/VLK. |
Images (3) Click the Picture to Zoom In
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ARG10736 anti-Laminin 111 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat brain stem stained with ARG10736 anti-Laminin 111 antibody (red) at 1:1000 dilution and costained with ARG52344 anti-Myelin Basic Protein antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)
The Laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.
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ARG10736 anti-Laminin 111 antibody IHC-Fr image
Immunohistochemistry: Frozen sections of Mouse cortex stained with ARG10736 anti-Laminin 111 antibody (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.
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ARG10736 anti-Laminin 111 antibody WB image
Western blot: 1) Rat heart cells lysates, and 2) 0.2 µg of purified laminin-111 protein from Mouse EHS sarcoma was stained with ARG10736 anti-Laminin 111 antibody. This antibody recognizes 3 laminin isotypes: α1 (440 kDa), β1 (220kD) and γ1 (220 kDa). Also recognized a laminin binding protein at 120 kDa in both Rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this cross reactivity is irrelevant.
Specific References
Alcohol-Induced Alterations in the Vascular Basement Membrane in the Substantia Nigra of the Adult Human Brain
IHC-P / Human
Alginate-Laminin Hydrogel Supports Long-Term Neuronal Activity in 3D Human Induced Pluripotent Stem Cell-Derived Neuronal Networks
ICC/IF / Human
