ARG57145
anti-Lactate Dehydrogenase B antibody [14D7]
anti-Lactate Dehydrogenase B antibody [14D7] for Western blot and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody
Overview
| Product Description | Mouse Monoclonal antibody [14D7] recognizes Lactate Dehydrogenase B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 14D7 |
| Isotype | IgG2a, kappa |
| Target Name | Lactate Dehydrogenase B |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-334 of Human Lactate Dehydrogenase B |
| Conjugation | Un-conjugated |
| Alternate Names | Renal carcinoma antigen NY-REN-46; LDH heart subunit; TRG-5; LDHBD; LDH-H; EC 1.1.1.27; LDH-B; HEL-S-281; L-lactate dehydrogenase B chain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LDHB |
| Gene Full Name | lactate dehydrogenase B |
| Background | This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010] |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody |
| Calculated MW | 37 kDa |
Images (1) Click the Picture to Zoom In
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ARG57145 anti-Lactate Dehydrogenase B antibody [14D7] WB image
Western blot: 50 ng of 1) Recombinant LDHB protein, 40 µg of 2) A549, 3) 293T, 4) K562, and 5) Jurkat cell lysates stained with ARG57145 anti-Lactate Dehydrogenase B antibody [14D7] at 1:1000.
