ARG65177
anti-LSP1 antibody
anti-LSP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes LSP1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LSP1 |
| Antigen Species | Human |
| Immunogen | C-RYKFVATGHGKYEKV |
| Conjugation | Un-conjugated |
| Alternate Names | Lymphocyte-specific protein 1; pp52; 52 kDa phosphoprotein; 47 kDa actin-binding protein; WP34; Lymphocyte-specific antigen WP34 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 37 kDa |
| PTM | Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis (By similarity). |
Images (4) Click the Picture to Zoom In
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ARG65177 anti-LSP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65177 anti-LSP1 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG65177 anti-LSP1 antibody WB image
Western blot: 35 µg of Human peripheral blood mononucleocyte (A), lymph node (B) and tonsil (C) lysates (in RIPA buffer) stained with ARG65177 anti-LSP1 antibody at 0.05 µg/ml (A), 0.03 µg/ml (B, C) dilutions and incubated at RT for 1 hour.
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ARG65177 anti-LSP1 antibody WB image
Western blot: 35 µg of Daudi cell lysate (in RIPA buffer) stained with ARG65177 anti-LSP1 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
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ARG65177 anti-LSP1 antibody WB image
Western blot: 35 µg of Mouse spleen lysate (in RIPA buffer) stained with ARG65177 anti-LSP1 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
