ARG66441
anti-LRAT antibody
anti-LRAT antibody for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes LRAT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LRAT |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 80-160 of Human LRAT. |
| Conjugation | Un-conjugated |
| Alternate Names | Phosphatidylcholine--retinol O-acyltransferase; Lecithin retinol acyltransferase; LCA14; EC 2.3.1.135 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~ 25 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LRAT |
| Gene Full Name | lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) |
| Background | The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014] |
| Function | Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments. [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein. Rough endoplasmic reticulum. Endosome, multivesicular body. Cytoplasm, perinuclear region. Note=Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells (By similarity). [UniProt] |
| Calculated MW | 26 kDa |
Images (2) Click the Picture to Zoom In
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ARG66441 anti-LRAT antibody ICC/IF image
Immunofluorescence: HUVEC cells stained with ARG66441 anti-LRAT antibody. The picture on the right is blocked with the synthetic peptide.
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ARG66441 anti-LRAT antibody WB image
Western blot: COLO205 cell lysate stained with ARG66441 anti-LRAT antibody at 1:500 dilution.
