ARG63216
anti-LIS1 / PAFAH1B1 antibody
anti-LIS1 / PAFAH1B1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes LIS1 / PAFAH1B1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LIS1 / PAFAH1B1 |
| Antigen Species | Human |
| Immunogen | TGSVDQTVKVWECR |
| Conjugation | Un-conjugated |
| Alternate Names | Platelet-activating factor acetylhydrolase IB subunit alpha; LIS-1; MDCR; LIS1; LIS2; PAF acetylhydrolase 45 kDa subunit; PAF-AH 45 kDa subunit; PAF-AH alpha; Lissencephaly-1 protein; PAFAH; MDS; PAFAH alpha |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P43034 Human Platelet-activating factor acetylhydrolase IB subunit alpha Swiss-port # P63004 Rat Platelet-activating factor acetylhydrolase IB subunit alpha |
|---|---|
| Background | This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq, Apr 2009] |
| Research Area | Neuroscience antibody |
| Calculated MW | 47 kDa |
Images (4) Click the Picture to Zoom In
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ARG63216 anti-LIS1 / PAFAH1B1 antibody WB image
Western Blot: Rat Ovary lysate (35 µg protein in RIPA buffer) stained with ARG63216 anti-LIS1 / PAFAH1B1 antibody at 0.1 µg/ml dilution.
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ARG63216 anti-LIS1 / PAFAH1B1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63216 anti-LIS1 / PAFAH1B1 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63216 anti-LIS1 / PAFAH1B1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63216 anti-LIS1 / PAFAH1B1 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63216 anti-LIS1 / PAFAH1B1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63216 anti-LIS1 / PAFAH1B1 antibody at 3.75 µg/ml dilution followed by AP-staining.
