ARG56576
anti-LIPE / HSL antibody
anti-LIPE / HSL antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes LIPE / HSL |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Multiple isoforms of LIPE / HSL are known to exist. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LIPE / HSL |
| Immunogen | Synthetic peptide (14 aa) within aa. 920-970 of Human LIPE / HSL. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.1.1.79; AOMS4; HSL; LHS; Hormone-sensitive lipase; FPLD6 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Human lymph node tissue. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LIPE |
| Gene Full Name | lipase, hormone-sensitive |
| Background | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008] |
| Function | In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. [UniProt] |
| Calculated MW | Isoform 1: 117 kDa Isoform 2: 84 kDa |
| PTM | Phosphorylation by AMPK may block translocation to lipid droplets. |
Images (3) Click the Picture to Zoom In
-
ARG56576 anti-LIPE / HSL antibody IF image
Immunofluorescence: Human lymph node cells stained with ARG56576 anti-LIPE / HSL antibody at 20 µg/ml dilution.
-
ARG56576 anti-LIPE / HSL antibody IHC-P image
Immunohistochemistry: Human lymph node tissue stained with ARG56576 anti-LIPE / HSL antibody at 2.5 µg/ml dilution.
-
ARG56576 anti-LIPE / HSL antibody WB image
Western blot: Human lymph node tissue lysate stained with ARG56576 anti-LIPE / HSL antibody at (A) 0.5 and (B) 1 µg/ml dilution.
