ARG54122
anti-LIN28A antibody
anti-LIN28A antibody for Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes LIN28A |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | LIN28A |
| Antigen Species | Human |
| Immunogen | Purified recombinant human LIN28A protein fragments expressed in E.coli |
| Conjugation | Un-conjugated |
| Alternate Names | LIN28; Protein lin-28 homolog A; LIN-28; Lin-28A; CSDD1; Zinc finger CCHC domain-containing protein 1; ZCCHC1; lin-28A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 26 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.2% Sodium azide and 50% Glycerol |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 3.75 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LIN28A |
| Gene Full Name | lin-28 homolog A (C. elegans) |
| Background | Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis.Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and,indirectly,in stabilizing the mRNAs.Binds IGF2 mRNA,MYOD1 mRNA,ARBP/36B4 ribosomal protein mRNA and its own mRNA.Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression By similarity.Acts as a suppressor of microRNA(miRNA)biogenesis by specifically binding the precursor let-7(pre-let-7),a miRNA precursor.Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase,leading to the terminal uridylation of pre-let-7.Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation.Degradation of pre-let-7 in embryonic stem(ES)cells contributes to the maintenance of ES cells.In contrast,LIN28A down-regulation in neural stem cells by miR-125,allows the processing of pre-let-7.Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7.Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. |
| Function | Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus |
| Research Area | Gene Regulation antibody |
| Calculated MW | 23 kDa |
Images (1) Click the Picture to Zoom In
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ARG54122 anti-LIN28A antibody WB image
Western blot: NTERA cell lysate stained with ARG54122 anti-LIN28A antibody at 1:1000 dilution.
