ARG64962
anti-LIG1 / DNA ligase 1 antibody
anti-LIG1 / DNA ligase 1 antibody for Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes LIG1 / DNA ligase 1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Rat |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LIG1 / DNA ligase 1 |
| Antigen Species | Human |
| Immunogen | C-RVREDKQPEQATTS |
| Conjugation | Un-conjugated |
| Alternate Names | EC 6.5.1.1; DNA ligase 1; Polydeoxyribonucleotide synthase [ATP] 1; DNA ligase I |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 102 kDa |
Images (1) Click the Picture to Zoom In
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ARG64962 anti-LIG1 / DNA ligase 1 antibody WB image
Western blot: HeLa nuclear lysate (35 µg protein in RIPA buffer) stained with ARG64962 anti-LIG1 / DNA ligase 1 antibody at 0.1 µg/ml dilution.
