ARG40375
anti-LDHA / Lactate Dehydrogenase antibody
anti-LDHA / Lactate Dehydrogenase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes LDHA / Lactate Dehydrogenase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LDHA / Lactate Dehydrogenase |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to A2-R106 of Human LDHA. |
| Conjugation | Un-conjugated |
| Alternate Names | PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59; HEL-S-133P; L-lactate dehydrogenase A chain; GSD11; LDHM; EC 1.1.1.27; LDH-M; LDH-A |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 37 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LDHA |
| Gene Full Name | lactate dehydrogenase A |
| Background | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody |
| Calculated MW | 37 kDa |
| PTM | ISGylated. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG40375 anti-LDHA / Lactate Dehydrogenase antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG40375 anti-LDHA / Lactate Dehydrogenase antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG40375 anti-LDHA / Lactate Dehydrogenase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine stained with ARG40375 anti-LDHA / Lactate Dehydrogenase antibody at 1 µg/ml dilution.
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ARG40375 anti-LDHA / Lactate Dehydrogenase antibody WB image
Western blot: Rat spleen, ANA-1 and Jurkat whole cell lysates stained with ARG40375 anti-LDHA / Lactate Dehydrogenase antibody at 0.5 µg/ml dilution.
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ARG40375 anti-LDHA / Lactate Dehydrogenase antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal Goat serum and then stained with ARG40375 anti-LDHA / Lactate Dehydrogenase antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG40375 anti-LDHA / Lactate Dehydrogenase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine stained with ARG40375 anti-LDHA / Lactate Dehydrogenase antibody at 1 µg/ml dilution.
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ARG40375 anti-LDHA / Lactate Dehydrogenase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue stained with ARG40375 anti-LDHA / Lactate Dehydrogenase antibody at 1 µg/ml dilution.
