ARG57101
anti-LDHA / Lactate Dehydrogenase antibody [1A4]
anti-LDHA / Lactate Dehydrogenase antibody [1A4] for Flow cytometry,ICC/IF,Western blot and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody
Overview
| Product Description | Mouse Monoclonal antibody [1A4] recognizes LDHA / Lactate Dehydrogenase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A4 |
| Isotype | IgG2b, kappa |
| Target Name | LDHA / Lactate Dehydrogenase |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-332 of Human LDHA |
| Conjugation | Un-conjugated |
| Alternate Names | PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59; HEL-S-133P; L-lactate dehydrogenase A chain; GSD11; LDHM; EC 1.1.1.27; LDH-M; LDH-A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LDHA |
| Gene Full Name | lactate dehydrogenase A |
| Background | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008] |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody |
| Calculated MW | 37 kDa |
| PTM | ISGylated. |
Images (4) Click the Picture to Zoom In
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ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] at 1:500 (Red).
Hoechst 3342 (Blue) for nucleus staining.
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ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] FACS image
Flow Cytometry: HeLa cell line stained with ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] WB image
Western blot: 40 µg of 1) Jurkat, 2) 293T, and 3) A431 cell lysates stained with ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] at 1:1000.
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ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] at 1:8000.
