ARG44394

anti-LCTL antibody

anti-LCTL antibody for Western blot,Flow cytometry and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes LCTL
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Target Name LCTL
Antigen Species Human
Immunogen Human LCTL recombinant protein (aa. sequence: D67-Q537).
Conjugation Un-conjugated
Alternate Names LCTL; Lactase Like; KLPH; KLG; Klotho/Lactase-Phlorizin Hydrolase-Related Protein; KL Lactase Phlorizin Hydrolasel; Lactase-Like Protein

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6 cells
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 197021 Human LCTL

GeneID: 235435 Mouse LCTL

Swiss-port # Q6UWM7 Human Lactase-like protein

Swiss-port # Q8K1F9 Mouse Lactase-like protein

Gene Symbol LCTL
Gene Full Name Lactase Like
Background This gene encodes a member of family 1 glycosidases. Glycosidases are enzymes that hydrolyze glycosidic bonds and are classified into families based on primary amino acid sequence. Most members of family 1 have two conserved glutamic acid residues, which are required for enzymatic activity. The mouse ortholog of this protein has been characterized and has a domain structure of an N-terminal signal peptide, glycosidase domain, transmembrane domain, and a short cytoplasmic tail. It lacks one of the conserved glutamic acid residues important for catalysis, and its function remains to be determined (PMID: 12084582). Alternative splicing results in multiple transcript variants.
Function Plays a role in formation of the lens suture in the eye, which is important for normal optical properties of the lens.
Cellular Localization Endoplasmic reticulum, Membrane
Calculated MW 65 kDa
PTM Glycoprotein

Images (4) Click the Picture to Zoom In

  • ARG44394 anti-LCTL antibody WB image

    Western blot: A549, A431 and MCF-7 stained with ARG44394 anti-LCTL antibody at 0.5 μg/mL dilution.

  • ARG44394 anti-LCTL antibody FACS image

    Flow Cytometry: HepG2 stained with ARG44394 anti-LCTL antibody at 1 μg/10^6 cells dilution.

  • ARG44394 anti-LCTL antibody WB image

    Western blot: C6 stained with ARG44394 anti-LCTL antibody at 0.5 μg/mL dilution.

  • ARG44394 anti-LCTL antibody WB image

    Western blot: Neuro-2a stained with ARG44394 anti-LCTL antibody at 0.5 μg/mL dilution.