ARG62530
anti-L1CAM antibody [UJ127]
anti-L1CAM antibody [UJ127] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [UJ127] recognizes L1CAM |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | UJ127 |
| Isotype | IgG1 |
| Target Name | L1CAM |
| Antigen Species | Human |
| Immunogen | Homogenous suspension of 16 week human fetal brain |
| Conjugation | Un-conjugated |
| Alternate Names | SPG1; CD171; NCAM-L1; MASA; MIC5; S10; CAML1; HSAS1; N-CAM-L1; CD antigen CD171; N-CAML1; HSAS; Neural cell adhesion molecule L1 |
Application Instructions
| Application Note | IHC: 1 - 2 µg/ml IP: 2 µg/mg of lysate WB: 1 - 2 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | IMR-5 cells |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | L1CAM |
| Gene Full Name | L1 cell adhesion molecule |
| Background | The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013] |
| Function | Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. [UniProt] |
| Cellular Localization | Cell membrane |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 140 kDa |
Images (1) Click the Picture to Zoom In
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ARG62530 anti-L1CAM antibody [UJ127] WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG62530 anti-L1CAM antibody [UJ127] at 1:500 dilution.
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Review for anti-L1CAM antibody [UJ127]
Application：WB
Sample：HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
EphB regulates L1 phosphorylation during retinocollicular mapping.
IP /
The dimensions and characteristics of the subepidermal nerve plexus in human skin--terminal Schwann cells constitute a substantial cell population within the superficial dermis.
ICC/IF / Human
Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.
WB / Human
Two alcohol binding residues interact across a domain interface of the L1 neural cell adhesion molecule and regulate cell adhesion.
FACS / Human
Modification of the L1-CAM carboxy-terminus in pancreatic adenocarcinoma cells.
IHC / Human
