ARG62528
anti-Ku 70 / Ku 80 antibody [162]
anti-Ku 70 / Ku 80 antibody [162] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Monkey,Xenopus laevis
Cancer antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [162] recognizes Ku 70 / Ku 80 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk, Xenopus laevis |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 162 |
| Isotype | IgG2a |
| Target Name | Ku 70 / Ku 80 |
| Antigen Species | Human |
| Immunogen | Human B cell nuclei from plasmacytoid 2p68 cells |
| Conjugation | Un-conjugated |
| Alternate Names | DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| Positive Control | HeLa cells and tonsil tissue. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified Antibody |
| Buffer | 1X PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P12956 Human X-ray repair cross-complementing protein 6 Swiss-port # P23475 Mouse X-ray repair cross-complementing protein 6 |
|---|---|
| Gene Symbol | XRCC6 |
| Gene Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| Background | The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008] |
| Function | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody |
| Calculated MW | 70 kDa |
| PTM | Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. |
Clone References
Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.
WB / Human
Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends.
IP / Human
Identification and functional analysis of a human homologue of the monkey replication origin ors8.
ChIP / Human, Primates
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
GSA / Human
Analysis of the DNA replication competence of the xrs-5 mutant cells defective in Ku86.
FACS / Hamster
In vivo association of Ku with mammalian origins of DNA replication.
IP / Primates
Elevated breast cancer risk in irradiated BALB/c mice associates with unique functional polymorphism of the Prkdc (DNA-dependent protein kinase catalytic subunit) gene.
WB / Mouse, Pig
Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro.
IHC / Human
Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens.
ICC/IF, IHC-P, Puri, FACS / Human, Monkey, Mouse, Xenopus laevis, Rat
