ARG40904
anti-Ki-67 antibody
anti-Ki-67 antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Microvascular Density Study antibody; Proliferation Marker antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Ki-67 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Ki-67 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human Ki-67. |
| Conjugation | Un-conjugated |
| Alternate Names | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| Positive Control | Ramos |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MKI67 |
| Gene Full Name | marker of proliferation Ki-67 |
| Background | Ki-67 is a nuclear protein. It is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] |
| Function | Ki-67 required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt] |
| Cellular Localization | Chromosome. Nucleus, nucleolus. [UniProt] |
| Research Area | Microvascular Density Study antibody; Proliferation Marker antibody |
| Calculated MW | 359 kDa |
| PTM | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG40904 anti-Ki-67 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG40904 anti-Ki-67 antibody.
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ARG40904 anti-Ki-67 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG40904 anti-Ki-67 antibody.
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ARG40904 anti-Ki-67 antibody WB image
Western blot: Ramos cell lysate stained with ARG40904 anti-Ki-67 antibody.
