ARG63985
anti-KPNB1 antibody
anti-KPNB1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes KPNB1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KPNB1 |
| Antigen Species | Human |
| Immunogen | C-RRSKTNKAKTLAT |
| Conjugation | Un-conjugated |
| Alternate Names | Pore targeting complex 97 kDa subunit; Importin-90; Importin subunit beta-1; IPOB; Nuclear factor p97; PTAC97; Impnb; Karyopherin subunit beta-1; NTF97; IPO1; IMB1 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq, Jul 2008] |
| Research Area | Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 97 kDa |
| PTM | Mono-ADP-ribosylated by PARP16. |
Images (2) Click the Picture to Zoom In
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ARG63985 anti-KPNB1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63985 anti-KPNB1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63985 anti-KPNB1 antibody WB image
Western Blot: Daudi cell lysate (35 µg protein in RIPA buffer) stained with ARG63985 anti-KPNB1 antibody at 0.03 µg/ml dilution.
