ARG63401
anti-KPNA2 / IPOA1 antibody
anti-KPNA2 / IPOA1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes KPNA2 / IPOA1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KPNA2 / IPOA1 |
| Antigen Species | Human |
| Immunogen | C-QVQDGAPGTFNF |
| Conjugation | Un-conjugated |
| Alternate Names | Karyopherin subunit alpha-2; SRP1alpha; Importin subunit alpha-1; QIP2; RCH1; IPOA1; SRP1-alpha; RAG cohort protein 1 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 58 kDa |
Images (3) Click the Picture to Zoom In
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ARG63401 anti-KPNA2 / IPOA1 antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG63401 anti-KPNA2 / IPOA1 antibody at 1:1000 dilution.
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ARG63401 anti-KPNA2 / IPOA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63401 anti-KPNA2 / IPOA1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63401 anti-KPNA2 / IPOA1 antibody WB image
Western blot: 35 µg of HeLa cell lysate (in RIPA buffer) stained with ARG63401 anti-KPNA2 / IPOA1 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
Customer's Feedback
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Review for anti-KPNA2 / IPOA1 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
