ARG54750
anti-KMT1B / SUV39H2 antibody
anti-KMT1B / SUV39H2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KMT1B / SUV39H2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | KMT1B / SUV39H2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 93-122 (N-terminus) of Human SUV39H2 (NP_001180353.1). |
| Conjugation | Un-conjugated |
| Alternate Names | H3-K9-HMTase 2; KMT1B; Lysine N-methyltransferase 1B; Histone-lysine N-methyltransferase SUV39H2; EC 2.1.1.43; Su; Histone H3-K9 methyltransferase 2; var; Suppressor of variegation 3-9 homolog 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HepG2 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9EQQ0 Mouse Histone-lysine N-methyltransferase SUV39H2 Swiss-port # Q9H5I1 Human Histone-lysine N-methyltransferase SUV39H2 |
|---|---|
| Gene Symbol | SUV39H2 |
| Gene Full Name | suppressor of variegation 3-9 homolog 2 (Drosophila) |
| Function | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation. [UniProt] |
| Cellular Localization | Nucleus. Chromosome, centromere. Note=Associates with centromeric constitutive heterochromatin. |
| Research Area | Gene Regulation antibody |
| Calculated MW | 47 kDa |
Images (2) Click the Picture to Zoom In
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ARG54750 anti-KMT1B / SUV39H2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma tissue stained with ARG54750 anti-KMT1B / SUV39H2 antibody.
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ARG54750 anti-KMT1B / SUV39H2 antibody WB image
Western blot: 35 µg of HepG2 cell lysate stained with ARG54750 anti-KMT1B / SUV39H2 antibody at 1:1000 dilution.
