ARG66361

anti-KHK (isoform C) antibody

anti-KHK (isoform C) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes KHK (isoform C)
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity The antibody detects endogenous level of total KHK Isoform C protein.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name KHK (isoform C)
Antigen Species Human
Immunogen Synthetic peptide around aa. 102-106 (NNSNG) of Human KHK (isoform C).
Conjugation Un-conjugated
Alternate Names Hepatic fructokinase; Ketohexokinase; EC 2.7.1.3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3795 Human KHK

Swiss-port # P50053 Human Ketohexokinase

Gene Symbol KHK
Gene Full Name ketohexokinase (fructokinase)
Background This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Function Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate. [UniProt]
Calculated MW 33 kDa

Images (2) Click the Picture to Zoom In

  • ARG66361 anti-KHK (isoform C) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded tumors derived from Huh-7 cells stained with ARG66361 anti-KHK (isoform C) antibody (left), or the same antibody pre-incubated with KHK-C aa. 72-115 peptide (right).

  • ARG66361 anti-KHK (isoform C) antibody WB image

    Western blot: Hepatocytee, Hep3B and Huh7 cell lysates stained with ARG66360 anti-KHK (isoform A) antibody (upper panel) and ARG66361 anti-KHK (isoform C) antibody (lower panel).