ARG56686

anti-KGF antibody

anti-KGF antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes KGF
Tested Reactivity Hu, Rat
Tested Application ELISA, IHC-P, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name KGF
Antigen Species Human
Immunogen E.coli derived Recombinant Human KGF.
(CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT)
Conjugation Un-conjugated
Alternate Names FGF-7; Fibroblast growth factor 7; Heparin-binding growth factor 7; KGF; Keratinocyte growth factor; HBGF-7

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56795 as a detection antibody
IHC-P0.5 µg/ml
Neut8.0 µg/ml (To yield [ND50] of the biological activity of hKGF (18.9 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2252 Human FGF7

GeneID: 29348 Rat FGF7

Swiss-port # P21781 Human Fibroblast growth factor 7

Swiss-port # Q02195 Rat Fibroblast growth factor 7

Gene Symbol FGF7
Gene Full Name fibroblast growth factor 7
Background The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]
Function Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. [UniProt]
Calculated MW 23 kDa

Images (4) Click the Picture to Zoom In

  • ARG56686 anti-KGF antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal Human skin. The recommended ARG56686 anti-KGF antibody concentration is 0.5 µg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.

  • ARG56686 anti-KGF antibody WB image

    Western blot: 250 - 0.24 ng of Human KGF stained with ARG56686 anti-KGF antibody, under reducing conditions.

  • ARG56686 anti-KGF antibody WB image

    Western blot: 250 - 0.24 ng of Human KGF stained with ARG56686 anti-KGF antibody, under non-reducing conditions.

  • ARG56686 anti-KGF antibody standard curve image

    Sandwich ELISA: ARG56686 anti-KGF antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56795 anti-KGF antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.