ARG59524
anti-KDM6B / JMJD3 antibody
anti-KDM6B / JMJD3 antibody for ICC/IF,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KDM6B / JMJD3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KDM6B / JMJD3 |
| Antigen Species | Mouse |
| Immunogen | Recombinant fusion protein corresponding to aa. 90-190 of Mouse KDM6B / JMJD3 (NP_001017426.1). |
| Conjugation | Un-conjugated |
| Alternate Names | JmjC domain-containing protein 3; Lysine-specific demethylase 6B; JMJD3; Lysine demethylase 6B; Jumonji domain-containing protein 3; EC 1.14.11.- |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | SH-SY5Y | ||||||
| Observed Size | 170 - 180 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | KDM6B |
|---|---|
| Gene Full Name | lysine (K)-specific demethylase 6B |
| Function | Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 177 kDa |
Images (2) Click the Picture to Zoom In
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ARG59524 anti-KDM6B / JMJD3 antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells stained with ARG59524 anti-KDM6B / JMJD3 antibody at 1:100 dilution.
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ARG59524 anti-KDM6B / JMJD3 antibody WB image
Western blot: 25 µg of SH-SY5Y cell lysates stained with ARG59524 anti-KDM6B / JMJD3 antibody at 1:3000 dilution.
