ARG40596

anti-KDEL antibody [10C3]

anti-KDEL antibody [10C3] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bird,Insect,Mammal,Plant,Yeast

Overview

Product Description Mouse Monoclonal antibody [10C3] recognizes KDEL
Tested Reactivity Hu, Ms, Rat, Bird, Insect, Mamm, Plnt, Yeast
Tested Application ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 10C3
Isotype IgG2a
Target Name KDEL
Antigen Species Rat
Immunogen Synthetic peptide corresponding to aa. 649-654 (SEKDEL) of Rat Grp78.
Conjugation Un-conjugated
Alternate Names Lys-Asp-Glu-Leu; Lysine-aspartic acid-glutamate-leucine

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 µg/ml
IHC-P5 - 10 µg/ml
WB4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol.
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Calculated MW 25 kDa

Images (3) Click the Picture to Zoom In

  • ARG40596 anti-KDEL antibody [10C3] ICC/IF image

    Immunofluorescence: C2C12 myoblasts transfected with wild-type mouse ADAM12 and stained with ARG40596 anti-KDEL antibody [10C3] at 10 µg/ml dilution.

  • ARG40596 anti-KDEL antibody [10C3] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human spleen tissue stained with ARG40596 anti-KDEL antibody [10C3] at 10 µg/ml dilution.

  • ARG40596 anti-KDEL antibody [10C3] WB image

    Western blot: Grp78 (BiP) recombinant protein, RK-13, Mouse liver microsomes, Rat liver microsomes and Heat-shocked HeLa cell lysates stained with ARG40596 anti-KDEL antibody [10C3].