ARG64817
anti-KCNJ1 / ROMK antibody
anti-KCNJ1 / ROMK antibody for Western blot and Human
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes KCNJ1 / ROMK |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize reported isoforms NP_722449.2 and NP_000211. The following reported variants represent identical protein: NP_722451.1, NP_722449.2, NP_722450.1, NP_722448.1. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KCNJ1 / ROMK |
| Antigen Species | Human |
| Immunogen | C-DQININFVVDAGNEN |
| Conjugation | Un-conjugated |
| Alternate Names | ATP-sensitive inward rectifier potassium channel 1; KIR1.1; Inward rectifier K; Potassium channel, inwardly rectifying subfamily J member 1; ROMK; ATP-regulated potassium channel ROM-K; ROMK1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P48048 Human ATP-sensitive inward rectifier potassium channel 1 |
|---|---|
| Background | Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 45 kDa |
| PTM | Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane. |
Images (1) Click the Picture to Zoom In
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ARG64817 anti-KCNJ1 / ROMK antibody WB image
Western Blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG64817 anti-KCNJ1 / ROMK antibody at 1 µg/ml dilution.
