ARG52400
anti-KCC2 / Potassium Chloride Cotransporter phospho (Ser940) antibody
anti-KCC2 / Potassium Chloride Cotransporter phospho (Ser940) antibody for Western blot and Rat
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KCC2 / Potassium Chloride Cotransporter phospho (Ser940) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Bov, Dog, NHuPrm |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KCC2 / Potassium Chloride Cotransporter |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | K-Cl cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Solute carrier family 12 member 5; hKCC2; KCC2; Neuronal K-Cl cotransporter |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~135k KCC2 protein phosphorylated at Ser940. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KCC2 |
| Gene Full Name | solute carrier family 12 (potassium-chloride transporter), member 5 |
| Background | KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007). |
| Research Area | Neuroscience antibody |
| Calculated MW | 126 kDa |
Images (1) Click the Picture to Zoom In
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ARG52400 anti-KCC2 / Potassium Chloride Cotransporter phospho (Ser940) antibody WB image
Western blot: Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control) stained with ARG52400 anti-KCC2 / Potassium Chloride Cotransporter phospho (Ser940) antibody.
Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide).
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