ARG65753
anti-K-Ras antibody
anti-K-Ras antibody for Western blot and Human,Mouse,Rat
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes K-Ras |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Specificity | Based on the sequence homologous information, this antibody might cross-react to H-Ras (92%) and N-Ras (91%). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | K-Ras |
| Antigen Species | Human |
| Immunogen | Recombinant protein around aa. 30-189 of Human K-Ras. |
| Conjugation | Un-conjugated |
| Alternate Names | NS3; KRAS1; KRAS2; RALD; K-RAS4A; GTPase KRas; c-Ki-ras; KI-RAS; RASK2; K-RAS2B; K-RAS2A; K-RAS4B; c-K-ras; C-K-RAS; NS; CFC2; Ki-Ras; K-Ras 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRAS |
| Gene Full Name | Kirsten rat sarcoma viral oncogene homolog |
| Background | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. [UniProt] |
| Calculated MW | 22 kDa |
| PTM | Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). |
Images (1) Click the Picture to Zoom In
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ARG65753 anti-K-Ras antibody WB image
Western blot: 30 µg of HepG2, MEF, Mouse brain, and Rat brain lysates stained with ARG65753 anti-K-Ras antibody at 1:500 dilution.
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Review for anti-K-Ras antibody
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
