ARG51531
anti-JunB phospho (Ser259) antibody
anti-JunB phospho (Ser259) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes JunB phospho (Ser259) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | JunB |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from Human JunB. |
| Conjugation | Un-conjugated |
| Alternate Names | AP-1; Transcription factor jun-B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | JUNB |
| Gene Full Name | jun B proto-oncogene |
| Background | Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. |
| Function | Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30207 Phospho JunB Antibody Duo (Total, pSer259) Related products: JunB antibodies; JunB Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: JunB as a novel gate keeper for prostate cancer progression |
| Research Area | Gene Regulation antibody |
| Calculated MW | 36 kDa |
| PTM | Ubiquitinated by ITCH, leading to its degradation. |
Images (2) Click the Picture to Zoom In
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ARG51531 anti-JunB phospho (Ser259) antibody WB image
Western blot: Extracts from HT29 cells untreated or treated with EGF stained with ARG51531 anti-JunB phospho (Ser259) antibody.
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ARG51531 anti-JunB phospho (Ser259) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51531 anti-JunB phospho (Ser259) antibody (left) or the same antibody preincubated with blocking peptide (right).
