ARG22706
anti-Integrin alpha E2 antibody [OX-62]
anti-Integrin alpha E2 antibody [OX-62] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Rat
Overview
| Product Description | Mouse Monoclonal antibody [OX-62] recognizes Integrin alpha E2 This antibody recognizes OX-62 antigen, also known as rat alpha E2 integrin, an 1150 amino acid, ~130 kDa single pass type I transmembrane protein expressed by intestinal dendritic cells, dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of dendritic morphology in lymphoid organs, at sites where gamma delta T cells are present. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | FACS, IHC-Fr, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | OX-62 |
| Isotype | IgG1 |
| Target Name | Integrin alpha E2 |
| Antigen Species | Rat |
| Immunogen | Density gradient enriched PVG rat thoracic duct dendritic cells. |
| Conjugation | Un-conjugated |
| Alternate Names | CD103; HUMINAE; Integrin alpha-E; Mucosal lymphocyte 1 antigen; CD antigen CD103; HML-1 antigen; Integrin alpha-IEL |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | ITGAE |
|---|---|
| Gene Full Name | integrin, alpha E |
| Background | Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008] |
| Function | Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. [UniProt] |
| Calculated MW | 130 kDa |
