ARG62525
anti-ITK antibody [2F12]
anti-ITK antibody [2F12] for Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [2F12] recognizes ITK |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2F12 |
| Isotype | IgG1 |
| Target Name | ITK |
| Immunogen | amino-terminal peptide of Itk |
| Conjugation | Un-conjugated |
| Alternate Names | LPFS1; Interleukin-2-inducible T-cell kinase; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; PSCTK2; EMT; Kinase EMT; Tyrosine-protein kinase Lyk; LYK; IL-2-inducible T-cell kinase; EC 2.7.10.2 |
Application Instructions
| Application Note | WB: 1/1000 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Buffer | 1X PBS buffer with <0.1% sodium azide. |
| Preservative | <0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITK |
| Gene Full Name | IL2-inducible T-cell kinase |
| Background | This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008] |
| Function | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 72 kDa |
| PTM | Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain. Ubiquitinated. |
