ARG64308
anti-ITCH / AIF4 antibody
anti-ITCH / AIF4 antibody for Flow cytometry,ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ITCH / AIF4 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | FACS, ICC/IF, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ITCH / AIF4 |
| Antigen Species | Human |
| Immunogen | C-EIKSHDLKPNGGN |
| Conjugation | Un-conjugated |
| Alternate Names | EC 6.3.2.-; Itch; Atrophin-1-interacting protein 4; ADMFD; E3 ubiquitin-protein ligase Itchy homolog; NFE2-associated polypeptide 1; AIF4; AIP4; NAPP1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96J02 Human E3 ubiquitin-protein ligase Itchy homolog |
|---|---|
| Background | This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 103 kDa |
| PTM | On T-cell activation, phosphorylation by the JNK cascade on serine and threonine residues surrounding the PRR domain accelerates the ubiquitination and degradation of JUN and JUNB. The increased ITCH catalytic activity due to phosphorylation by JNK1 may occur due to a conformational change disrupting the interaction between the PRR/WW motifs domain and the HECT domain and, thus exposing the HECT domain (By similarity). Phosphorylation by FYN reduces interaction with JUNB and negatively controls JUN ubiquitination and degradation. Ubiquitinated; autopolyubiquitination with 'Lys-63' linkages which does not lead to protein degradation. |
Images (4) Click the Picture to Zoom In
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ARG64308 anti-ITCH / AIF4 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG64308 anti-ITCH / AIF4 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64308 anti-ITCH / AIF4 antibody WB image
Western blot: 35 µg of Human brain (frontal cortex) lysate (in RIPA buffer) stained with ARG64308 anti-ITCH / AIF4 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG64308 anti-ITCH / AIF4 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG64308 anti-ITCH / AIF4 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG64308 anti-ITCH / AIF4 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64308 anti-ITCH / AIF4 antibody (green) at 10 µg/ml dilution for 1 hour. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
