ARG44685
anti-IRF9 antibody
anti-IRF9 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes IRF9 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | IRF9 |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | ISGF-3 gamma; Transcriptional regulator ISGF3 subunit gamma; ISGF3G; ISGF3; Interferon-stimulated gene factor 3 gamma; IRF-9; ISGF3 p48 subunit; p48; IFN-alpha-responsive transcription factor subunit; Interferon regulatory factor 9 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purification |
| Buffer | PBS with 0.09% sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IRF9 |
| Gene Full Name | interferon regulatory factor 9 |
| Background | This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Mutations in this gene result in Immunodeficiency 65. [provided by RefSeq, Jul 2020] |
| Function | Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta. [UniProt] |
| Calculated MW | 44 kDa |
| PTM | Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity. Polyubiquitinated by TRAF6 after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated; leading to nuclear translocation. [UniProt] |
Images (3) Click the Picture to Zoom In
-
ARG44685 anti-IRF9 antibody IHC-P image
Immunohistochemistry: Human Lung stained with ARG44685 anti-IRF9 antibody at 5 µg/mL dilution.
-
ARG44685 anti-IRF9 antibody WB image
Western blot: A431 and THP1 stained with ARG44685 anti-IRF9 antibody at 1 µg/mL dilution.
-
ARG44685 anti-IRF9 antibody IP image
Immunoprecipitation: THP1 lysate immunoprecipitated with 2.5 µg of ARG44685 anti-IRF9 antibody.
