ARG65197
anti-IQGAP1 antibody
anti-IQGAP1 antibody for Western blot and Human
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes IQGAP1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IQGAP1 |
| Antigen Species | Human |
| Immunogen | C-KRQSGQTDPLQKEE |
| Conjugation | Un-conjugated |
| Alternate Names | SAR1; Ras GTPase-activating-like protein IQGAP1; HUMORFA01; p195 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P46940 Human Ras GTPase-activating-like protein IQGAP1 |
|---|---|
| Background | This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 189 kDa |
| PTM | Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. |
Images (1) Click the Picture to Zoom In
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ARG65197 anti-IQGAP1 antibody WB image
Western Blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG65197 anti-IQGAP1 antibody at 1 µg/ml dilution.
