ARG66734
anti-INTS5 antibody
anti-INTS5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes INTS5 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | INTS5 |
| Antigen Species | Human |
| Immunogen | Recombinant full length protein of Human INTS5. |
| Conjugation | Un-conjugated |
| Alternate Names | INT5; KIAA1698; Integrator complex subunit 5; Int5 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | MCF7, HT29 and Mouse liver | ||||||
| Observed Size | ~ 120 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | INTS5 |
| Gene Full Name | integrator complex subunit 5 |
| Background | The Integrator complex is a complex that associates with the C-terminal domain of RNA polymerase II large subunit. This complex is brought to U1 and U2 small nuclear RNA genes, where it is involved in the transcription and processing of their transcripts. The protein encoded by this gene represents a subunit of the Integrator complex. [provided by RefSeq, Aug 2016] |
| Function | Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex (PubMed:23904267). [UniProt] |
| Cellular Localization | Nucleus membrane; Multi-pass membrane protein. Nucleus. Cytoplasm. [UniProt] |
| Calculated MW | 108 kDa |
Images (2) Click the Picture to Zoom In
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ARG66734 anti-INTS5 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human esophageal cancer tissue section. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was stained with ARG66734 anti-INTS5 antibody at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
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ARG66734 anti-INTS5 antibody WB image
Western blot: MCF7, HT29 and Mouse liver whole cell lysates stained with ARG66734 anti-INTS5 antibody.
