ARG40388
anti-IMPDH2 antibody
anti-IMPDH2 antibody for ICC/IF,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IMPDH2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IMPDH2 |
| Antigen Species | Human |
| Immunogen | Recombinant full length protein of Human IMPDH2. |
| Conjugation | Un-conjugated |
| Alternate Names | IMPD2; EC 1.1.1.205; IMPDH-II; IMP dehydrogenase 2; IMPD 2; Inosine-5'-monophosphate dehydrogenase 2; IMPDH 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 56 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P12268 Human Inosine-5'-monophosphate dehydrogenase 2 Swiss-port # P24547 Mouse Inosine-5'-monophosphate dehydrogenase 2 |
|---|---|
| Gene Symbol | IMPDH2 |
| Gene Full Name | IMP (inosine 5'-monophosphate) dehydrogenase 2 |
| Background | This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 56 kDa |
| PTM | The N-terminus is blocked. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG40388 anti-IMPDH2 antibody ICC/IF image
Immunofluorescence: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG40388 anti-IMPDH2 antibody (red) in 3% BSA-PBS and incubated overnight at 4°C.
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ARG40388 anti-IMPDH2 antibody WB image
Western blot: HeLa, PC3, Mouse spleen and Mouse heart lysates stained with ARG40388 anti-IMPDH2 antibody.
