ARG56793

anti-IL7 antibody (Biotin)

anti-IL7 antibody (Biotin) for ELISA,Western blot and Rat

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes IL7
Tested Reactivity Rat
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL7
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat IL-7.
(MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT CWNKILKGSI)
Conjugation Biotin
Alternate Names IL-7; Interleukin-7

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: ~ 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56684 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25647 Rat IL7

Gene Symbol Il7
Gene Full Name interleukin 7
Background The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]
Function Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. [UniProt]
Calculated MW 20 kDa

Images (2) Click the Picture to Zoom In

  • ARG56793 anti-IL7 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Rat IL-7 stained with ARG56793 anti-IL7 antibody (Biotin), under reducing conditions.

  • ARG56793 anti-IL7 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Rat IL-7 stained with ARG56793 anti-IL7 antibody (Biotin), under non-reducing conditions.