ARG66703

anti-IL6R alpha antibody

anti-IL6R alpha antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes IL6R alpha
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL6R alpha
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human IL6R alpha.
Conjugation Un-conjugated
Alternate Names IL-6R-alpha; CD126; IL6RA; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL6Q; CD antigen CD126; IL-6R 1; IL-6RA; gp80; IL6RQ; Interleukin-6 receptor subunit alpha; IL-6R-1; Membrane glycoprotein 80

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:300
Application Note IHC-P: Antigen Retrieval: EDTA buffer (pH 8.0) at high pressure and temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3570 Human IL6R

Swiss-port # P08887 Human Interleukin-6 receptor subunit alpha

Gene Symbol IL6R
Gene Full Name interleukin 6 receptor
Background This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.[provided by RefSeq, May 2011]
Function Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.

Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. [UniProt]
Cellular Localization Isoform 1: Basolateral cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt]
Calculated MW 52 kDa
PTM A short soluble form may also be released from the membrane by proteolysis. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG66703 anti-IL6R alpha antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: EDTA buffer (pH 8.0) at high pressure and temperature. The tissue section was stained with ARG66703 anti-IL6R alpha antibody at 1:200 diution, overnight at 4°C.