ARG21471

anti-IL6 antibody [MP5-20F3]

anti-IL6 antibody [MP5-20F3] for ELISA,ELISPOT,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Neutralizing and Mouse

Overview

Product Description Rat Monoclonal antibody [MP5-20F3 ] recognizes IL6
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, ICC/IF, IHC-Fr, IHC-P, IP, Neut
Specificity Mouse IL-6.
Host Rat
Clonality Monoclonal
Clone MP5-20F3
Isotype IgG1, kappa
Target Name IL6
Antigen Species Mouse
Immunogen COS-expressed mouse IL-6
Conjugation Un-conjugated
Alternate Names B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA< 1:250
ELISPOTAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P1:10 - 1:100
IPAssay-dependent
NeutAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16193 Mouse IL6

Swiss-port # P08505 Mouse Interleukin-6

Gene Symbol IL6
Gene Full Name interleukin 6
Background This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt]
Highlight Related products:
IL6 antibodies; IL6 ELISA Kits; IL6 recombinant proteins; Anti-Rat IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Calculated MW 24 kDa
PTM N- and O-glycosylated.

Images (1) Click the Picture to Zoom In

  • ARG21471 anti-IL6 antibody [MP5-20F3] standard curve image

    ELISA: Mouse IL-6 detected by ARG21471 anti-IL6 antibody [MP5-20F3] as capture antibody ( ARG21472: Azide free version), and ARG21474 anti-IL6 antibody [MP5-32C11] (Biotin) as detection antibody, follow by incubation with streptavidin-HRP.