ARG56736

anti-IL6 antibody (Biotin)

anti-IL6 antibody (Biotin) for ELISA,Western blot and Human

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes IL6
Tested Reactivity Hu
Tested Application ELISA, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IL6
Antigen Species Human
Immunogen E.coli derived Recombinant Human IL-6.
(PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM)
Conjugation Biotin
Alternate Names B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1 µg/ml
Sandwich: 0.1 - 1 µg/ml
WB0.1 - 0.2 µg/ml
Application Note Sandwich ELISA (Capture antibody - Detection antibody):
ARG56591 (1 - 2 µg/ml) - ARG56736 (0.1 - 0.2 µg/ml)
ARG56627 (0.5 - 2 µg/ml) - ARG56736 (0.25 - 1 µg/ml)

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3569 Human IL6

Swiss-port # P05231 Human Interleukin-6

Gene Symbol IL6
Gene Full Name interleukin 6
Background This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt]
Highlight Related products:
IL6 antibodies; IL6 ELISA Kits; IL6 recombinant proteins; Anti-Goat IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Calculated MW 24 kDa
PTM N- and O-glycosylated.

Images (3) Click the Picture to Zoom In

  • ARG56736 anti-IL6 antibody (Biotin) standard curve image

    Direct ELISA: ARG56736 anti-IL6 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56736 anti-IL6 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56591 anti-IL6 antibody [1.2-2B11-2G10] as a capture antibody at 1 - 2 µg/ml dilution combined with ARG56736 anti-IL6 antibody (Biotin) as a detection antibody at 0.1 - 0.2 µg/ml dilution. Results of a typical standard run with optical density.

  • ARG56736 anti-IL6 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56627 anti-IL6 antibody as a capture antibody at 0.5 - 2 µg/ml dilution combined with ARG56736 anti-IL6 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml dilution. Results of a typical standard run with optical density.