ARG56591
anti-IL6 antibody [1.2-2B11-2G10]
anti-IL6 antibody [1.2-2B11-2G10] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [1.2-2B11-2G10] recognizes IL6 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ELISA, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1.2-2B11-2G10 |
| Isotype | IgG1, kappa |
| Target Name | IL6 |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human IL-6. (PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM) |
| Conjugation | Un-conjugated |
| Alternate Names | B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2 |
Application Instructions
| Application Suggestion |
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| Application Note | Sandwich ELISA (Capture antibody - Detection antibody): ARG56591 (1 - 2 µg/ml) - ARG56736 (0.1 - 0.2 µg/ml) ARG56591 (1 - 2 µg/ml) - ARG56627 (0.1 - 0.2 µg/ml) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL6 |
| Gene Full Name | interleukin 6 |
| Background | This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011] |
| Function | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt] |
| Highlight | Related products: IL6 antibodies; IL6 ELISA Kits; IL6 recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| Calculated MW | 24 kDa |
| PTM | N- and O-glycosylated. |
Images (4) Click the Picture to Zoom In
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ARG56591 anti-IL6 antibody [1.2-2B11-2G10] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56591 anti-IL6 antibody [1.2-2B11-2G10] concentration is 0.125 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56591 anti-IL6 antibody [1.2-2B11-2G10] WB image
Western blot: 250 - 0.24 ng (left to right) of Human IL-6 stained with ARG56591 anti-IL6 antibody [1.2-2B11-2G10], under reducing conditions.
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ARG56591 anti-IL6 antibody [1.2-2B11-2G10] standard curve image
Sandwich ELISA: ARG56591 anti-IL6 antibody [1.2-2B11-2G10] as a capture antibody at 1 - 2 µg/ml dilution combined with ARG56736 anti-IL6 antibody (Biotin) as a detection antibody at 0.1 - 0.2 µg/ml dilution. Results of a typical standard run with optical density.
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ARG56591 anti-IL6 antibody [1.2-2B11-2G10] WB image
Western blot: 250 - 0.24 ng (left to right) of Human IL-6 stained with ARG56591 anti-IL6 antibody [1.2-2B11-2G10], under non-reducing conditions.
Specific References
Ultrasensitive Detection of Interleukin 6 by Using Silicon Nanowire Field-Effect Transistors
ELISA / Human
