ARG66001

anti-IL5 antibody (Biotin)

anti-IL5 antibody (Biotin) for ELISA,Western blot and Human

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes IL5
Tested Reactivity Hu
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL5
Antigen Species Human
Immunogen E. coli derived recombinant Human IL5.
(MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES)
Conjugation Biotin
Alternate Names Eosinophil differentiation factor; EDF; IL-5; TRF; T-cell replacing factor; B-cell differentiation factor I; Interleukin-5

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66000 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3567 Human IL5

Swiss-port # P05113 Human Interleukin-5

Gene Symbol IL5
Gene Full Name interleukin 5
Background This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. [UniProt]
Calculated MW 15 kDa

Images (2) Click the Picture to Zoom In

  • ARG66001 anti-IL5 antibody (Biotin) standard curve image

    Direct ELISA: ARG66001 anti-IL5 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66001 anti-IL5 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG66001 anti-IL5 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66000 anti-IL5 antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.