ARG66429
anti-IL4 antibody [SQab18131]
anti-IL4 antibody [SQab18131] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [SQab18131] recognizes IL4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab18131 |
| Isotype | IgG1, kappa |
| Target Name | IL4 |
| Antigen Species | Human |
| Immunogen | Recombinant Human IL4 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Affinity purification with immunogen. |
|---|---|
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL4 |
| Gene Full Name | interleukin 4 |
| Background | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 17 kDa |
Images (4) Click the Picture to Zoom In
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ARG66429 anti-IL4 antibody [SQab18131] ICC/IF image
Immunofluorescence: MCF-7 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66429 anti-IL4 antibody [SQab18131] (green) at 1:400 and cell nuclei were stained with Hoechst 33342 (blue).
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ARG66429 anti-IL4 antibody [SQab18131] FACS image
Flow Cytometry: MCF-7 cells were stained with ARG66429 anti-IL4 antibody [SQab18131] at 1 µg/ml (red) and without antibody control (black).
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ARG66429 anti-IL4 antibody [SQab18131] WB image
Western blot: 100 ng of Recombinant IL4 protein and 60 µg of 293T cell lysate stained with ARG66429 anti-IL4 antibody [SQab18131] at 1:2000 dilution.
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ARG66429 anti-IL4 antibody [SQab18131] ELISA image
ELISA: Titration curve of ARG66429 anti-IL4 antibody [SQab18131]. Red: IL4; Black: BSA (negative control).
