ARG56726
anti-IL4 antibody (Biotin)
anti-IL4 antibody (Biotin) for ELISA,Western blot and Rat
Overview
| Product Description | Biotin-conjugated Rabbit Polyclonal antibody recognizes IL4 |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IL4 |
| Antigen Species | Rat |
| Immunogen | E.coli derived Recombinant Rat IL-4. (MHGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS) |
| Conjugation | Biotin |
| Alternate Names | BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Il4 |
| Gene Full Name | interleukin 4 |
| Background | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. [UniProt] |
| Calculated MW | 17 kDa |
Images (4) Click the Picture to Zoom In
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ARG56726 anti-IL4 antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Rat IL-4 stained with ARG56726 anti-IL4 antibody (Biotin), under reducing conditions.
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ARG56726 anti-IL4 antibody (Biotin) standard curve image
Direct ELISA: ARG56726 anti-IL4 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.
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ARG56726 anti-IL4 antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Rat IL-4 stained with ARG56726 anti-IL4 antibody (Biotin), under non-reducing conditions.
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ARG56726 anti-IL4 antibody (Biotin) standard curve image
Sandwich ELISA: ARG56726 anti-IL4 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56611 anti-IL4 antibody as a capture antibody. Results of a typical standard run with optical density.
