ARG56611

anti-IL4 antibody

anti-IL4 antibody for ELISA,Western blot and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes IL4
Tested Reactivity Rat
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL4
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat IL-4.
(MHGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS)
Conjugation Un-conjugated
Alternate Names BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56726 as a detection antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 287287 Rat IL4

Swiss-port # P20096 Rat Interleukin-4

Gene Symbol Il4
Gene Full Name interleukin 4
Background The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Function Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. [UniProt]
Calculated MW 17 kDa

Images (3) Click the Picture to Zoom In

  • ARG56611 anti-IL4 antibody WB image

    Western blot: 250 - 0.24 ng of Rat IL-4 stained with ARG56611 anti-IL4 antibody, under reducing conditions.

  • ARG56611 anti-IL4 antibody WB image

    Western blot: 250 - 0.24 ng of Rat IL-4 stained with ARG56611 anti-IL4 antibody, under non-reducing conditions.

  • ARG56611 anti-IL4 antibody standard curve image

    Sandwich ELISA: ARG56611 anti-IL4 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56726 anti-IL4 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.